Copyright 1995-2001 S.H. Shakman,  Institute Of Science; all rights reserved.
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      Serial dilution method:   "Separation of staphylococci, E.

coli and saprophytic streptococci from the specifically virulent

streptococci in nasopharyngeal swabbings was accomplished in serial

dilution cultures in dextrose-brain broth using a nicrome wire at

steps of 10-2, 10-6 and 10-10 in which the specifically virulent

streptococci outgrow the avirulent streptococci, staphylococci and

E. coli." [55R1]


  Regarding a number of diseases, including glaucoma, epilepsy,

MS., epidemic poliomyelitis and coronary heart disease:  "The

number of colonies and percentage incidence of isolation of hte

streptococcus from the liver, spleen and kidney without evidence of

lesions were uniformly high regardless of the source of the

streptococcus, indicating it would seem a protective scavenger-like

function of these organs."


  In 1955 Dr. Rosenow noted that a "remarkable elective or specific

localization occured in organs of mice corresponding to those

chiefly involved in patients from whom the streptococcus was

isolated from the nasopharynx ..."

  However, when 14 specific strains were mixed and stored at 10

degrees C for 71 days in glycerin-NaCl solution, and then grown in

dextrose-brain broth and injected intravenously in laboratary mice,

not only had characteristic specificities disappeared, but also

"the number of streptococci and percentage incidence of isolations

from the different organs of mice were almost without exception far

greater than what would be expected ... [comprising a] remarkable

increase in localizing property or 'virulence'".  Moreover, results

indicated that "each streptococcus that remained viable in the

stored composite glycerine-NaCl solution suspension had acquired

the diverse localizing properties characteristic of the 14

respective specific strains. ... Most remarkable of all are the

facts (1) that the changes in the composite mixture of the specific

types of streptococci occurred at a temperature of 10 degrees C and

other conditions that precluded growth, (2) that the newly acquired

properties were transmissible as the streptococci grew in

subculture in the dextrose brain broth, and (3) that such changes

never occurred under comparable conditions of storage of specific

strains separately."

  "The maintenance almost indefinitely of respective specificities

on storage separately of specific strains of alpha streptococci as

partially dehydrated in the glycerol-NaCl solution menstruum nd the

phenomenal increase in organotropic localization on additional

identical storage of composite mixtures of respective specific

strains of streptocci is new, truly remarkable and fundamental, for

it indicates the importance of environmental conditions for the

acquisition and maintenance of respective specifities of the ever-

present alpha streptocci in human beings.  The importance of

determining specifities inherent or acquired of alpha type

streptococci in studies on etiology such as these is obvious."

[55R1, p. 246]

[Go to ROSENOW Bibliography]